Re: cytoplasmic CD3 or cytoplasmic CD22

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>
> I would like to post the following message on the flow cytometry
> listserv:
>
> Does anyone routinely use cytoplasmic CD3 or cytoplasmic CD22 for acute
> leukemias? If so, what has been your experience? Are these markers
> helpful? We are considering adding these to our panel.
>
> Tim Singleton, MD
> singleto@umich.edu
>
Hi, Tim, regarding your question on intracytoplasmic CD3 and CD22, I am
routinely staining for these antigens in combination with
myeloperoxidase. We are using Caltag's FIX & PERM reagents for fixation
and permeabilization and have good experience.

Elisabeth Paietta

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu