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>
> I am looking for advise on a stain/dye that will penetrate cells
> (specifically bacteria) or bind with the surface membrane and not
> have to be washed off before acquisition. The stain/dye must excite
> with a 488nm laser and need either no fixation or a fixative that is
> mixed with the dye and does not need washing before acquisition.
The quickest way would be to use the SYTO cell permeant nucleic acid stains.
For example, we use SYTO BC from Molecular Probes (comes in the
Bacterial Counting Kit).
_______________________________________________
Dr Daniel Deere (ddeere@rna.bio.mq.edu.au)
Royal Society Research Fellow
School of Biological Sciences,
Macquarie University,
Sydney,
Australia NSW 2109.
Tel- +61 2 9850 8150/8238 or 98685510
Fax- +61 2 9850 8245
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