Fluorescein-labeled Peanut Agglutinin(PNA)

YKJEONG@amherst.edu
Tue, 29 Oct 1996 00:16:58 -0500 (EST)

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Hi! Flowers.
I have a plan to do flow cytometry of germinal center B cells stained
with Fluorescein-PNA. But, I do not have information about the suggested
final concentration and amount of the reagent for staining 1 x 10e6 cells.
I got the Fluorescein-PNA (stock conc. 5mg/ml) from "Calbiochem".
If anyone have done this kind of work, please give some tips for my work.
Thank you for your attention.
Sincerely,

Youngkee Jeong
Hybridoma Lab.
Biology Dept.
Amherst College
Ykjeong@amherst.edu
(FAX)(413)542-7955

Next message: Keith Bahjat: "Signal to Noise"
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu