 |
 |
 |
 |
I agree that it would be nice to have an ideal panel for granulocytes but it
would not include IgE for the purpose of discriminating eosinophils from
basophils. Human eosinophils but not mouse have low affinity receptors
(CD23) on their surfaces (J. Immunol. 143:3580; Immunol. Res: 11:252; J. Allergy
Clin. Immunol. 94(part 2. suppl S):1214) for IgE. MPO is good for PMNs,
eosinophils have light scatter and autofluorescence, and basophil should
have a different light scatter pattern owing to the extremely large granules
they contain. In as much as I do not work with basophils I do not know if they
have an unique or almost unique surface marker that would allow their
discrimination.
Steve Kayes
==========================
Stephen G. Kayes, Ph.D Ofc: (334) 460-6768
Professor FAX: (334) 460-6771
Structural and Cellular Biology; 2042 MSB
University of South Alabama College of Medicine
Mobile, AL 36688-0002
E-mail: kayes@sungcg.usouthal.edu
==========================
>>>>>>>>>>>>>>>>>>>>>>Original Message Follows>>>>>>>>>>>>>>>>>>>
Dear Andreas,
If nothing specific comes to mind with respect to the CD surface antigens and
you do not want to discriminate on the bases of elastical light scatter, an
optional staining would be one that reacts with:
MPO (specific for neutrophils)
EPO (specific for eosinophils)
IgE (specific for basophils)
Robbert van Leeuwen
Applied Optics / Flow Cytometry Group
University of Twente
The Netherlands
|
|
|
|