FACSCalibur parameter settings for analysis of baacteria
Dyane N Sonier (dsonier@wsunix.wsu.edu)
Thu, 24 Oct 1996 17:07:49 -0700 (PDT)
I am a graduate student working on my MS. I would like to use flow
cytometry to quantify viable cells in various applications. I am
currently working on using flow cytometry to analyze isopropanol killed
(or fixed) E. coli cells stained with: Molecular Probes' BacLight Bacteria
Viability Staining Kit (adapted for flow cytometry), Ethidium Bromide, or
Propidium Iodide. I have had great difficulty acquiring events from
stained cell samples that do not look the same as a stain + water only
sample. I have used FACSComp to set the machine settings, and am using
FSC=E02 Lin. After varying parameter settings, looking for a stained
bacterial population, I was/am still unable to "find" bacteria with or
without gating. I would greatly appreciate any suggestions for instrument
or parameter settings that would help me acquire data on bacteria, or any
comments on others' use of the BacLight Kit. Thank you for your time,
Dyane N. Sonier
Program in Environmental Science and Regional Planning
Washington State University
Pullman, WA 99164
(509)335-1051
e-mail: dsonier@wsunix.wsu.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
