dried plant material

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I want to determine to ploidy level of some dried herbous plants via
flowcytometry. I checked the material in the light microscope, and the
nuclei seemed to be intact. However, with flowcytometry (razor-blade
technique, DAPI) I found nothing but low intensity, non significant peaks.

My explanation for this behaviour is that not enough fluorochrome got
into the nucleus, but also after longer staining there was no change.

Has anyone got knowledge of a method to stain dried plant materials
(leaves)?

As I am new in the field of flowcytometry, please forgive me my
question if it seems stupid for you; _any_ help (or hint) is
appreciated.

Thanks in advance,

Wolfgang Wlach

Mag. Wolfgang Wlach Institut fuer Pharmakognosie
der Universitaet Wien
Pharmaziezentrum

Tel. +43/1/313 36/8270 Althanstr. 14
Fax. +43/1/313 36/772 1090 Wien

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu