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Our mouse anti CD3 antibody (FN18-FITC, BIOTIN and unlabelled) is now ava=
ilable. In the USA contact Biosource (fax (805-987-3385), in Europe Dr P =
v/d Meiden (fax:+31-15-284-3999 or e-mail:meiden@bprc.nl) at the BPRC in =
the Netherlands.
For CD4/CD8 ratio you should take CD4+CD3+/CD8+CD3+, and only the cells w=
ithin a lymphocyte gate. CD8+CD3- cells are "NK-like cells" and are somet=
imes more than 50% of your total CD8+ population in macaques.
Success
Henk Niphuis
--------------------------------------
Date: 16-10-1996 22:58
To: cyto-inbox
From: dladouceur
In a tube which combines CD2(FITC)/CD4(PE)/CD8(PerCP) mAb's, which=20
values are most appropriate to use to calculate the CD4:CD8 ratios=20
correctly. Would one use:
(i) the total %CD4 and the total %CD8 derived from single parameter =20=
histograms?=20
(ii) the %CD4+CD8- and the %CD8+CD4- derived from the CD4 vs CD8 dot =20=
plot quadrants?
(iii) Should one be using the %CD2+CD4+ and the %CD2+CD8+ values =20=
(Something that was just recently suggested to me)?
Thanks for all replies/insights in advance.
Diane Ladouceur
(613-957-0998)
=20
NB/ We were using CD2 instead of CD3, as this work is being done on=
=20
Cynomolgus monkeys, and at the time we had not yet identified a CD3=
mAb=20
which cross-reacted with this species.
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