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Jim,
Most procedures for TUNEL ("Tdt-Utp Nick End Labeling" - but I'm told that's
a misnomer anyway) are also designed to counterstain the DNA and have
ethanol as part of the fixation method, which can cause a loss of many
cell-surface antigens. Have them look at:
Sgonc R, Boeck G, Dietrich H, Gruber J, Recheis H, and Wick G: Simultaneous
determination of cell surface antigens and apoptosis. Trends in Genetics,
10:41-42, 1994.
The above uses a brief paraformaldehyde fixation followed by triton to
permeabilize and should work better to retain the surface. We have also had
success using the commercial product FACSLYSE from BD as a
fixation/peabilization reagent. See:
Syrjala MT, Tiirkainen M, Jansson SE, and Krusius T: Flow cytometric
analysis of terminal deoxynucleotidyl transferase. A simplified method.
American Journal of Clincial Pathology, 99:298-303, 1993.
Good luck,
Neal
-- Neal A. Benson Biotechnology Program, University of Florida E-mail: nbenson@biotech.ufl.edu
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