re: Intracellular pH and BCECF

Michael Fox (MFox@vines.ColoState.EDU)
Mon, 14 Oct 96 11:36:06 MDT

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Mario Roederer: "saturation binding & fluorescence intensity"
Previous message: Albert D Donnenberg, PhD: "To flow-ers in Western PA and environs"
Maybe in reply to: colette charland: "Intracellular pH and BCECF"

I have not used BCECF but have used ADB and SNARF extensively to measure
intracellular pH. Both of these dyes work on the ratio principle, actually
better than BCECF. For details on making ratio measurements for pH, you might
consult our publications on using ADB (Cytometry 9:441-447, 1988) and SNARF
(Cytometry 14:916-921, 1993). I hope these help out.

Mike Fox
Colorado State University

Next message: Mario Roederer: "saturation binding & fluorescence intensity"
Previous message: Albert D Donnenberg, PhD: "To flow-ers in Western PA and environs"
Maybe in reply to: colette charland: "Intracellular pH and BCECF"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu