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Frederick Garbrecht
Thomas Jefferson University
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> From: Walter Sharp <102675.320@compuserve.com>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Heat stability
> Date: Friday, October 11, 1996 12:49 AM
>
>
> The investigator mentioned by Jill Martin is quite right to refuse the
antibody.
>
> We here (Oman in the Persian Gulf) have had some experience with
overheating,
> given that the temp. can easily exceed 120 deg.
> We had a case recently where two antibodies were shipped by a company (I
wonder
> if it's the same one?) without ice.
> One was totally "dead" (CD55), one working but, to my mind, weakly so
(CD59).
> They were returned to sender who gave the same spiel about "we've never
had a
> problem before".
> The dead one was replaced, the weak one was sent back to us since it was
> "working".
> Surprise, surprise - it was totally dead by the time it reached us the
second
> time, only a few weeks later.
>
> The upshot is, in my view, the least one can expect is a shorter shelf
life of
> the more robust antibodies and a quick death for the fragile ones.
>
> I used to work for Coulter here in the middle east as their "specialist"
and
> there were many occasions where my troublshooting of customer problems
showed up
> inappropriate storage at site of use and/or transport of delicate "juice"
at
> ambient (120 + deg) temperatures.
>
> Wal Sharp
> Sultan Qaboos University
> Oman.
>
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