hematopoietic cell sizing by flow

Scott R Burger (burge009@gold.tc.umn.edu)
Fri, 11 Oct 96 13:06:18 -0500

Greetings, all --

Is anyone aware of studies done measuring the size of hematopoietic
progenitor cells as they differentiate? In other words, how do the
diameters of early cells, perhaps CD34+, HLA-DR-, differ from those of cells
at later stages of differentiation? Forward scatter should give a measure
of cell diameter, but has anyone calibrated this to microns? If this is not
known, would a fairly straightforward microbead study clear the matter up?

Thanks in advance for any assistance.

Scott

***************************************************************
Scott R. Burger, M.D.
Medical Director, Hematopoietic Cell Processing Laboratory
Assistant Medical Director, Blood Bank
Assistant Professor, Laboratory Medicine and Pathology
University of Minnesota Hospital and Clinic
burge009@gold.tc.umn.edu
612-626-4919
612-624-5411 (Fax)

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