bacteria and flow

knappt (knappt@aurorabio.com)
Fri, 11 Oct 1996 13:39:57 -0700

Hello all,

I am looking for references and/or advice on the following:

Staining live, unfixed bacteria with Hoechst 33342 (or another DNA stain =
with similar spectral characteristics). I tried loading bacteria with H =
33342 but they did not take up the dye. Do I need to use EDTA or some =
other agent to get the H 33342 into the bacteria?

Does anyone have any tips or good references in general on sorting =
bacteria using a flow cytometer.

Thanks in advance,

Tom Knapp
Aurora Biosciences Corporation
11149 N. Torrey Pines Road
La Jolla, California 92037 USA

email "knappt@aurorabio.com"
Web Site "http://www.aurorabio.com"
(619) 452-5000 ex 125

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu