RE: Dead cells pre-fixation

Mara Rocchi (M.ROCCHI@CGNET.COM)
Tue, 08 Oct 1996 10:37:00 -0700 (PDT)

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Protocol to use Ethidium Monoazide DNA staining to discriminate between live
and dead cells before fixation are reported in "Methods in cell biology,
Flow Cytometry" volume 41-42, second edition, Eds. Darzynkiewicz, Robinson
and Crissman, Academic Press.
Please tell us if it works or not.
Good luck,
Mara

Mara Rocchi
DVM, Ph.D.
International Livestock Research Institute
Nairobi, Kenya

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu