Re: your mail

Larry Seamer (LSEAMER@COBRA.UNM.EDU)
Fri, 27 Sep 1996 08:27:37 +0000

I can provide some information concerning the potential pitfalls part of
your question by refering you to a paper by Cinthia Hoy and myself from a
few years ago:

Thermal Denaturation of DNA for Immunochemical Staining of Incorporated
BrdUrd: Critical Factors That Affect the Amount of Fluorescence and the
Shape of BrdUrd/DNA Histogram: Cytometry 10:718-725 (1989).

On -1 xxx -1 SMTP%h82@aixterm1.urz.uni-heidelberg.de@COBRA.UNM.EDU wrote:

> Is anybody of you aware of a good (means working) protocol for
> a Br-desoxyuridine-FACS-procedure? I have used the B-D-protocol
> provided in their monoclonal antibody resource book, but without
> success :-(
> In particular I am looking for information about the FACS-setup
> for aquisition and analysis of cell proliferation and hints
> about possible pitfalls in the procedure.
>
> Any help is greatly appreciated.
>
>
> --
> Andreas Schr=F6der
>
>
>

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu