ca++ flux calibration
KUKURUGA@medmail.med.umich.edu
Tue, 24 Sep 1996 20:02:20 -0500 (EST)
We're attempting quantification fo Ca++ using indo1, and also using
MultiTime program. There are questions . . .
Some of the terms for the calibration equation are somewhat confusing:\
I think we have an approach for determining Rmax and Rmin, but
the R term . . . according to the manual, R is the fluor. intensity
ratio for the "experimental" Ca++ -- but, is that not a continuum as a
function of time? If this is true, R varies (unless R is in fact something
else)
Also . . . the correction term "Ssub-f2/Ssub-b2" . . . is the "ratio of
the blue or green fluorescence intensity of the calcium-free and bound
dye, respectively." How is this different from R?
I'm taking this directly from the MultiTime V3.0 manual . . . can't make
sense of it. Plus, I've read several papers describing the process --
all use a variation of the same calibration function. What am I missing?
Any help (explanations, or references which describe the logic behind
these terms, whatever . . . anyone have a web page with this technique
described?) will be greatly appreciated.
MAK.\
Mark A. KuKuruga
University of Michigan Flow Cytometry
kukuruga@medmail.med.umich.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
