DR+ activated T cells - how best to isolate?

• Messages sorted by: [ date ][ thread ][ subject ][ author ]
• Next message: Dennis_Young@CIS.ucsd.edu: "Labeled Virus"
• Previous message: AAllen1216@aol.com: "I have Elite Software problems too!"

I've been trying to isolate CD8+ activated T cells expressing DR
MHCII molecules on their surface using immunomagnetic beads. Unfortunately,
the beads don't readily separate from the cells afterwards in culture
overnight, and I often can't get enough cells for FACS analysis. Does
anyone know of a gentle way of coaxing lymphocytes off from immunomagnetic
beads - one that won't also alter the cells drastically, as I assume
trypsin would? I would greatly appreciate any comments; please send them
directly to :
mvp@nih.gov. Thaks for your time.

Mark Pavlick

• Next message: Dennis_Young@CIS.ucsd.edu: "Labeled Virus"
• Previous message: AAllen1216@aol.com: "I have Elite Software problems too!"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu