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>SYTOX Green resolves cells in the G1, S, and G2 phase of the cell cycle. It
>does so better than PI. We have not yet found a way of distinguishing late
>G2 phase cells from early prophase. Their DNA content is similar; only in
>metaphase the nucleus breaks up and individual chromosomes will be obtained
>(as sub-G1 particles). SYTOX Green works best with the methanol based
>fixation procedure I have outlined.
At the risk of appearing argumentative,..........
I would like to see data, published or otherwise, showing the improved
resolution described. The above statement implies SYTOX Green can resolve
late S phase from G2 and only has problems distinguishing late G2 phase
from mitotics, at least early mitotics. At least that it does so better
than PI. Can it resolve late S from late mitotics, telophase? I'm no fan
of PI and would be very interested in SYTOX Green, if it's better.
td
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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