Optical Filter QA

Geoffrey Osborne (Geoff.Osborne@anu.edu.au)
Mon, 5 Feb 1996 14:10:05 +1100

Hello,
I have just started a quality assurance program for the optical
filters used in out FACStar Plus, and have found that the spectral
characteristics of the filters in use are different from expected. As an
example I have created a Web page showng the transmission characteristics of
the two most commonly used filters from the FL1 and FL2 detectors on our
instrument. The URL is

http://jcsmr.anu.edu.au/facslab/filters.html

My questions are these: ( have a look at the gif's first..)
1. Is it normal to have those humps in the side of the transmission curves?
2. Is the 50% transmittance which is often quoted for a BP filter measured
to take into account those humps (see 530.gif), or lack there of (right side
of 575.gif)
3. If a filter is quoted at say 530/30 is it OK if the maximum transmittance
point is not in fact at 530, but below, or is this a sign of filter
deterioration?

Any input regarding this would be appreciated. Also I apologize to those
without WWW access, for cluttering up their email with this...
BUT, for those of you lucky enough to have it, and who aren't bored with the
murine eosinophil theme, I created an example WWW page of the usage of
polarised light, same URL as above but eos.html on the end.

Thanks
Geoff
======================================================================
Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|...
Phone: 61 6 249 3694
FAX: 61 6 249 2595
-----Surfing the Web?: Try http://jcsmr.anu.edu.au/facshome.html------
======================================================================

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