Re: Ethidium monoazide - Reply
PAUL@flowcyt.cyto.purdue.edu
Tue, 30 Jan 1996 08:13:48
> I wish to be able to identify dead cells in samples of fixed cells. The
> cells are HIV infected so they have to be fixed before running on the
> machine. I seem to remember ethidium azide can do this, has any one any
> experience of this dye? Who sells it? How do you use it?
>
> Simon Monard
> Aaron Diamond
> New York
Reply:
There is a method listed on page 174 of the Handbook of Flow
Cytometry Methods describing the use of ethidium monoazide -
essentially use it at 5ug/l and place under a while lamp for 10
minutes, wash and fix.
Regards
Paul
J.Paul Robinson, Purdue University Cytometry Labs
robinson@flowcyt.cyto.purdue.edu PH:317-494 6449 FAX:317-494 0517
web http://www.cyto.purdue.edu
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