autofluorescence

Antony Bakke (bakkea@ohsu.EDU)
Fri, 26 Jan 96 16:42:24 PST

We are having problems with high levels of autofluorescence in
murine spleen cells from transgenic animals. This shows up as a
cigar shape on an FL1 versus FL2 cytogram with NO antibody or any
fluorchrome present. The transgenics contain the alpha and beta
chain genes for human HLA-DR3.
This phenomena only occurs in the transgenic animals, not in liter
mates and not in other normal mouse strains. Any ideas?

Thanks in advance,
Tony Bakke

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu