 |
 |
 |
 |
______________________________ Reply Separator _________________________________
Subject: RE: Eosinophils
Author: p.openshaw@ic.ac.uk at INTERNET
Date: 25/01/96 18:30
In response to Janek Brozek:
> there is any antigen allowing POSITIVE selection and sorting of
> eosinophils... To my knowlege there's none, but ....
>
de Grooth et al (1987) Cytometry 8:539 used rotation of polarised light by
eosinophils to sort PNM and Eos on FALS/side scatter. A student in the lab
tried this with limited success, although the Abbott haematology analysers
apparently use this method routinely. Weil and Chused (1981) Blood 57:1099
used blue autofluorescence. Another similar method was used by Lehnert and
Steinkamp (1986) Cell Biophys 8:201 on rat pleural eos. Others select CD9+
16- granulocytes, which are mostly eos.
We would dearly love a good method, so please let me know what you decide
on.
*******************************************************************
*Peter Openshaw (p.openshaw@ic.ac.uk) Direct:0171 594 3854 *
*Respiratory Medicine Switch:+44 171 723 1252 x 3854/3 *
*St. Mary's Hosp. Med. School Fax:+44 171 724 7349 *
*Imperial College, Norfolk Place, London W2 1PG, UK *
|
|
|
|