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In message <199601050422.XAA04144@flowcyt.cyto.purdue.edu> writes:
> Regarding the use of the subdiploid PI method to assess apoptosis
> by flow cytometry:
>
> There are two cell preparatory methods: one analyzes whole cells and
> the other lyses the cells and analyzes the released nuclei.
>
> Gating is critical to determine an accurate percentage apoptotic.
>
> Each tube contains "live" cells, apoptotic cells, debris, and
> clumps.
>
> I use a whole cell method with a linear fluorescence scale and
> use both LS gates [mainly to exclude debris] and FL gates [mainly
> to exclude doublets and clumps].
>
_____________________________________________________________
/ Michael J. Herron University of MN, Dept. of Dermatology /
/ herro001@maroon.tc.umn.edu /
_____________________________________________________________
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