Re: sheath buffer ?

Susan D. DEMAGGIO (SDDEMAGG@uci.edu)
Mon, 08 Nov 93 08:26:24 PST

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Susan D. DEMAGGIO: "Re: sheath buffer ?"
Previous message: Yashpal Agrawal: "sheath buffer ?"
Maybe in reply to: Yashpal Agrawal: "sheath buffer ?"
Next in thread: Susan D. DEMAGGIO: "Re: sheath buffer ?"

Hello !
Could someone advise whether it is possible to use distilled water as the
sheath fluid on an FACStar, if sorting is not done. Are there any problems with
bacterial growth etc. Any experiences ?.
Please reply to me or on the net.
Thanks,
Yashpal Agrawal
Dept. of Clinical Chemistry,
Kuopio University Hospital,
POB1777, 70211 Kuopio, Finland

E-mail: yagrawal@messi.uku.fi
FAX:+358-71-173200

I use exclusively DI water with my EPICS for analysis, conserving the
Isoflow sheath fluid for sorting only. I have had no
problems with analysis or with contamination. I would
think the FACStar would be similar.

SUE DeMaggio
Developmental Biology Center
University of California, Irvine

Next message: Susan D. DEMAGGIO: "Re: sheath buffer ?"
Previous message: Yashpal Agrawal: "sheath buffer ?"
Maybe in reply to: Yashpal Agrawal: "sheath buffer ?"
Next in thread: Susan D. DEMAGGIO: "Re: sheath buffer ?"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu