 |
 |
 |
 |
We too have found that it is not possible to label the CD34+ selected cells
with anti-CD34, and we have not been able to convincingly detect mouse Ig on
the
cells either (I'm pretty sure it is a mouse monoclonal). We have tried holding
the cells at 37 degrees to let them re-express CD34
with only fair results (the usual problems with cell death and high
background).
We have convinced ourselves that we are indeed getting mostly CD34+ cells
because:
1) They form CFU's
2) They have the right light scatter pattern
3) They are markedly enriched for CD34 message by RT-PCR, and the non-adherent
cells
do not have detectable CD34 message.
I too would be interested in a good way to re-label the CD34+ cells - it would
be a heck of lot
easier than RT-PCR!!
|
|
|
|