AIS Celector Flasks
Dave Gebhard (gebhard@aecom.yu.edu)
Wed, 3 Nov 93 18:28:57 EST
I have a investigator using Celector Flasks for CD-34 enrichment.
After following the protocol, the cells are stained for CD 34 and run on
the FACScan. To date we have not seen any staining greater than background.
Question? Is it possible that the antibody on the flask is
remaining on the cells and blocking any subsequent staining?
We would like to develop the cells with a second step
reagent, but to what? Is the anti-CD 34 a mouse? rat? rabbit etc?
There has been no response from the company to date.
Dave Gebhard
Albert Einstein College of Medicine PH: 718-430-2724
FACS Facility, 348 Chanin Building FAX: 718-822-6538
1300 Morris Park Avenue
Bronx, New York 10461
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