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Along these same lines, if it were possible to tag a specific chromosome
within a mixture, do you have to use a counter stain to see all the other
unwanted chromosomes? I am concerned about the DNA fluorescence crossing over
into the tag fluorescence channel. My reasoning stems from the idea that you
could calculate the rate of coincidence based upon the ratio of the
desired chromosome to the undesired ones and adjust the flow rate to minimize
coincidence. Also, if you set the cytometer up with a bead which correlates
in fluorescence intensity with what you estimate as the fluorescence
equivalent to your tagged chromosome to maximize signal to noise, is this
one signal enough to sort by??? I realize these questions are somewhat
abstract, but if anyone has some experience with low-level fluorescence
detection and sorting combined, I would appreciate hearing about your
experiences.
Thanks,
--- ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Kristi R. Harkins, Lab Director <> Office: 515/294-2472 Cell & Hybridoma Facility <> Lab: 515/294-8504 Iowa State University <> FAX: 515/294-0453 1104 Molecular Biology Bldg. <> E-mail:kharkins@iastate.edu Ames, IA 50011 <> xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
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