lost data
jeff@sugen.sf.ca.us
Fri, 22 Oct 1993 08:55:22 -0800
I have just experienced a problem which I have never faced before. Using
LYSIS II and a BD FACScan, I collected data yesterday and saved it to a
Bernoulli disk. I was using PE-labelled second antibody, and I gated on
live cells using FSC/SSC. I collected 10K events (live gating), and
assumed that I was collecting FSC, SSC, FL1H, FL2H, and FL3H data. In
fact, I know that all my samples were positive for FL2H (except the
negative control), because I could see the data as I ran the samples.
However, today I tried to analyze the data saved on the Bernoulli, and
found to my surprise that only FSC, SSC, and FL1H data were available.
Also, the file size was 61K, where normally 10K events would result in a
file size of 104K. So apparently the FL2H and FL3H data were not saved.
Why not?
Has anyone else experienced this problem? And is there a way to recover my
data or is all hope lost?
jeff@sugen.sf.ca.us
******************
* Jeff Powell, Ph.D. *
* SUGEN Inc. *
* Redwood City *
* 415-306-7763 *
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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