Re: Quenching of fluorescein

Dave Coder (dave@nucleus.immunol.washington.edu)
Wed, 20 Oct 93 08:28:32 -0700

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One way has been to resuspend cells exposed to bacteria in a solution that contains
crystal violet which will quench fluorescein; ingested fluorescein-labeled bacteria
will stil fluoresce. Citations are in older literature 1982-1984. I can dig out some
if anyone is interested

Dave Coder
dcoder@u.washington.edu

Begin forwarded message:

Message-ID: <"70906191013991/3693 04061450*"@MHS>
To: list:;
Subject: Quenching of fluorescein

We want to measure phagocytosis of fluorescein-labeled bacter-
ia. To discriminate adhering from ingested particles we would

like to quench fluorescence of extracellular bacteria.

Is there any easy means besides use of a quenching anti-flou-
rescein antibody. A protocol or relevant citation of literature

would be gratefully acknowledged.

Please send response by E-mail. Thanks a lot.

Greetings from Frankfurt, Jo Betz.

------------------------------ End of forwarded message 1

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu