RE: NON-HUMAN PRIMATE FLOW CYTOMETRY
salmonm (salmonm@rheuma.bham.ac.uk)
Tue, 5 Oct 1993 10:20:34 +0100 (CET)
> In response to your question concerning staining of non-human primates for
> flow cytometery, Anne Brodie from the Yerkes Regional Primate Center in
> Atlanta, Georgia has informed me that the BD reagents Leu3a and Leu2a
> (anti CD4 and anti-CD8 respectively) work very well for the Great Apes as
> well as Pigtail and Stumptail macaques. However, their Leukogate product
> does not work very well. She has informed me that there might be some
> gating tricks in order to obtain optimal staining. Please give her a call
> at (404)727-7757 for further information.
>
> Good luck,
> Thomas Rowe, Centers for Disease Control and Prevention, Retrovirus
> Diseases Branch
> Atlanta, Georgia
I must have missed this earlier. If it is any use, the antibodies available
from Coulter: anti-2H4 (CD45RA) and 4B4 (CD29) which identify stages of T
cell differentiation, stand a very strong chance of working in non-human
primates, because they were both originally produced by immunization of mice
with cells from a non-human primate, namely Aotus trivirgatus, the owl
monkey
Have fun
Mike Salmon
E-mail: salmonm@rheuma.bham.ac.uk
Tel: 44 (0)21 414 6780
FAX: 44 (0)21 414 6794
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
