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>To: cytometry@flowcyt.cyto.purdue.edu
>Subject: A FACS related problem
>
>A certain molecule is expressed on the surface of two different cell lines at
>similar levels, but (probably) in two different conformations. I have a
>monoclonal that (probably!) discriminates between the two conformations. In
>comparing the staining pattern of these two cell lines I face the following
>problem.
>The (no first Ab) conjugate controls for these two cell lines stain at two
>different levels, so that it becomes impossible to compare the test
>stainings.
>
>1)If I change the voltage for one cell line to match its control staining to
>that of the other, won't it bring about a more dramatic (and unreal) effect
>on the test staining for this particular cell line?
>2)Or can I just superimpose the two control stainings and compare then compare
>the stainings with the monoclonal in question?
>If anyone could throw some light on this aspect of FACS data analysis I will
>be really greatful.
>Pl. send your replies at the following address:
>$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
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> SANJEEV KUMAR TEL>91-11-6863004
> IMMUNOBIOLOGY LABS FAX>91-11-6862125
> NATIONAL INSTITUTE OF IMMUNOLOGY TELEX>031-66183 NII IN
> ARUNA ASAF ALI MARG GRAMS>IMMUNOLOGY
> NEW DELHI-67 EMAIL> Sanjeev@NII.ernet.in
>
>$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
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>
I for one would also like to see some of the suggestions.
Perhaps they could be CC'd to the mailing list?
Cheers,
Sam Witherspoon
Pharmacology
Glaxo Inc
sw11527@usav01.glaxo.com
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