Sorting Biohazards

James Weaver 202-205-5037 (@VM.CC.PURDUE.EDU:WEAVER@FDACD)
Thu, 9 Sep 1993 08:39 EDT

This spring, at the Eastern Regional Coulter Elite Users Meeting, an abstract
was presented on this subject. It was : The Risk Assessment of Biohazards During

Sorting on an Epics Elite. by John Ferbas, J.B. Margolick, A Logar, K.R.
Chadwick, and C.R. Rinaldo. They tested for aerosol formation by sorting a
specimen containing a lytic bacteriophage and placed plates of target bacteria
in various places for detection of aerosols. They concluded that when negative
pressure was maintained inthe sorting chamber and the door was closed that ono
contamination of the external environment was detected. As expected, aerosols
were detected in the sorting chamber. They could be minimized by proper
alignment of the center stream and by negative air pressure in the sorting
chamber.
I hope this information is helpful in anwering questions about use of
biohazerdous materials.

Jim Weaver
Molecular Pharmacology
DRT, CDER, FDA
Washington, DC

V: 202-205-5037
E: WEAVER@FDACD.CDER.FDA.GOV
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