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SAMPLE DATA have been provided for trying out MFI at facilities where HP to
PC data file transfer is not available (self-unpacking file MFIDATA1.EXE).
A very detailed beginner's tutorial for these data is in preparation and
will be made available and announced here when ready.
MFI 3.4D requires 520K of free DOS memory. If you have less, you may wish
to wait for the next version in which memory requirements will again be
reduced.
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MFI 3.4D ENHANCEMENTS:
RUNS. Up to 16 'runs' are now remembered by MFI. A 'run' is a tagged
subset of the list mode data files. Each run may be given a one-line
description.
LABELS. Each list mode data file can now have a one-line descriptive label
written into it and displayed on MFI's run organization screen and text
output. The label keyword is customizable, so can be set to an FCS 2.0
public keyword, such as $COM (comments), or a private one, e.g. #LABEL.
****WARNING**** Despite the fact that the insertion of a label field into
the text block of an FCS1.0/2.0 data file is legal, USE OF MFI's LABEL
FEATURE COULD MAKE LIST MODE FILES UNREADABLE BY SOME NON-MFI SOFTWARE.
FACScan Research Software from 2/88 (HP 3.1) is able to read MFI-labeled
files without problems. READABILITY BY OTHER SOFTWARE IS UNKNOWN. You are
advised to keep backup copies of your data files unmodified by MFI until
tests are run. If your software is incompatible with MFI-labeled data
files, you can blank the default #LABEL keyword with the 'W' command on
MFI's run organization screen. This makes it difficult inadvertantly to
add a label.
SAVITZKY-GOLAY smoothing has been added. See the help sections on
histograms and peak detection for details.
KEYWORDS (see under bugs below for note on terminology) can now be
displayed for the file highlighted on the list on the run organization
screen. (In 3.4c they could be displayed only after results were
displayed.)
The TUTORIAL ('How to use MFI for the first time') has been modified to
incorporate the above new features. Those of you who keep a printed copy of
the tutorial on file should replace it with a printout of the new version.
Support was added for the Coulter Epics XL.
REGISTRATION is now required, free of charge, by email, in order to obtain
information on the amount of use MFI is getting, and the kinds of uses
people find most beneficial.
MEMORY REQUIREMENTS have grown from 460K (3.4a) to 520K for use with a
1000-dot dot plot. 2000 dots needs 550K. Memory requirements will be
reduced in a forthcoming release.
BUG FIXES:
F1 is now used uniformly throughout MFI to call help.
KEYWORDS. The "$/header" feature of 3.4c used terminology inconsistent with
the FCS 2.0 data file standard. In the standard, "header" refers to a
different portion of the file (containing the offsets). The portion
displayed by MFI 3.4c is termed the "text", and consists of "keywords" and
associated "values", e.g. the keyword $TOT with value 5000. Accordingly,
the display of this portion of the file is now called up with "K =
keywords".
Control-corrected median KMESF values for multiple peaks were reported
incorrectly.
Out-of-memory errors now produce a more helpful error message.
MFI.CFG files are now rejected when created by a newer version of MFI than
the version being executed.
---<END OF 3.4D ENHANCEMENTS>---
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz emartz@microbio.umass.edu
Professor of Immunology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
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