The MFI.EXE program includes extensive help including a tutorial. MFI
3.3f adds color to all text screens plus other minor enhancements and
bug fixes. These are listed in the file MFI33F.DOC available by
anonymous FTP as above.
For those new to the cytometry mailing list: MFI excels at printing a
compact table of corrected median fluorescence intensities from a
group of list mode files in batch runs (4 sec/file). Each run is
organized by tagging filenames on a master list, including gate
assignment (9 gates are remembered simultaneously), control marking,
and filename sequencing. Dot plots (used for graphic gate setting)
and optionally smoothed histograms (optionally overlayed) can be
viewed and printed. MFI works with files from most B-D and Coulter
cytometers.
If you find MFI useful, please let me know at
emartz@microbio.umass.edu. I would appreciate knowing your name,
position, institution, and cytometer model. I would especially like
to know whether, and for what purposes, you find MFI preferable to
your alternative software, and what alternative software you have
compared it to. Future enhancements depend on user feedback.
-- PLEASE NOTE CHANGE OF EMAIL ADDRESS /* - - - - - - - - - - - - - - - - - From - - - - - - - - - - - - - - - - - - Eric Martz emartz@microbio.umass.edu Professor of Immunology Voice 413-545-2325, FAX 413-545-1578 Morrill IVN Rm 203, Dept Microbiol, Univ Mass, Amherst MA 01003 USA - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - */
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