indo-1

• Messages sorted by: [ date ][ thread ][ subject ][ author ]
• Next message: vanburen%flovax.dnet@rocdec.roc.wayne.edu: "auto DNA analysis"
• Previous message: vanburen%flovax.dnet@rocdec.roc.wayne.edu: "PC Lysys (THE program)"

In reply to your question on March 3 about running indo-1:

We successfully run indo-1 with and without FITC and PE surface markers on a
BD FACS Vantage. Previously, we have had success with a BD FACStarPLUS and a
Coulter EPICS 753. I have also seen a successful demonstration of indo-1 on
a Coulter EPICS Elite. I haven't had a chance yet to see Coulter's new XL.
Also, we haven't tried it yet, but the Vantage is capable of indo-1 and 3
more (FITC, PE, PerCP/RED670) fluorochromes, as well as FCS, SSC, and time.

Of course, the easy part is running the samples on the cytometer. The hard
part is analyzing the data. What software are you currently using to analyze
indo-1?

Eric Van Buren vanburen%flovax.dnet@rocdec.roc.wayne.edu
Immunology & Microbiology 313 577 1009 voice
Wayne State University 313 577 1155 fax

• Next message: vanburen%flovax.dnet@rocdec.roc.wayne.edu: "auto DNA analysis"
• Previous message: vanburen%flovax.dnet@rocdec.roc.wayne.edu: "PC Lysys (THE program)"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu