frozen lymphocytes

auger@ux1.cso.uiuc.edu
Wed, 24 Feb 93 15:38:53 CST

Can anyone give me references to working with cell surface antigens (i.e.
CD4/CD8) on frozen/thawed lymphocytes? Is it possible without introducing
artifact? What kind of conditions (freezing/thawing) must be met to make
the procedure possible?

I imagine that there have been some published reports on the feasibility
of freezing isolated lymphocytes and then staining them with various
monoclonals, but quite frankly, if someone can send me their advice, it
saves me from going to the library!

I would appreciate any and all comments. Thanks.
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Julie A. Auger, Director 217-244-0559 voice
Flow Cytometry Facility 217-244-0466 fax
University of Illinois at Urbana-Champaign auger@ux1.cso.uiuc.edu
231 PABL, 1201 W. Gregory, Urbana, IL 61801
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu