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Gary Durack (gary@flowcyt.cyto.purdue.edu)
Fri, 12 Feb 93 08:49:08 GMT
I would appreciate hearing from anyone that has done bivariate
flow karyotyping of chromosomes using Chromomycin and Hoechst with
either an EPICS Elite or EPICS 752. In particular I am interested
in exactly how the "gated amp" trigger should be set up to acheive
the best results. FALS seems to be un unreliable trigger source
for this application and there will always be fluorescent emmision
from both the UV and 458nm beam at nearly the same wavelengths.
The gate trigger will only work properly on a "clean" signal that
is unique to the top beam. So my question is : what is the best
signal to use for this application?
gary durack
Purdue University Cytometry Laboratories
fax (317) 494-0517
phone (317) 494-8638
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
