Image Analysis - Help Please!
paul@vphb.vet.purdue.edu
Fri, 5 Feb 93 07:55:55 EST
- From Paul Robinson, Purdue University:
My group are in the process of making a significant purchase in the area of
image analysis instrumentation. Our studies have previously used flow cytometry
extensively
and will continue to do so, however, it is clear that there are many areas we
need to have a capability to do image analysis. I would appreciate some advice
from the group.
Our general needs are:
Evaluation of ion fluxes (Calcium etc,) pH
Oxidative burst such as HE, DCF, and similar
Membrane potential, fluidity studies
confocal microscopy, multiple color phenotyping, dual excitation & emission
Special options might be FRAP, cell-cell communication and similar
Video output, electronic image camera,
GOOD SOFTWARE ESSENTIAL (is this asking too much?)
There are a number of instruments out there that do many of the above. My
perception is that some systems can do lots of things but perhaps not so well
and
others can do, say, excellent confocal, but lack good software for ion flux
measurement.
I would appreciate advice from people who know something about particular
instruments or are image users to give me advice.
Instruments under consideration:
Biorad confocal
Leica Confocal
Meridian Instruments ACAS with confocal
and some others...... ????
Help please, this is our one opportunity to get some decent image equipment. I
don't want to blow it (too badly!). Call me, fax me, email me, help me......
Thanks, Paul Robinson PHONE: 317- 494 6449 FAX: 317-494 0517
Purdue University Cytometry Laboratories
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
