Non-activating CD's on T cells

Geoffrey W Osborne (gwo215@cscgpo.anu.edu.au)
Fri, 15 Jan 93 16:05:04 EST

Hi,
Just after a bit of feedback about intracellular cytokine staining
using saponin for permeablization. Basically is this the "go", and if so
does anybody know of a paper using it in a flow cytometer, as there are a few
papers around using it in Confocal microscopy. Any and all suggestions welcome.

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Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|... Splat!
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