CD4 enumeration

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1) Is there documentation as to what actually happens to the CD4 receptor
and/or the cell(s) bearing these receptors, once the specimen is subject to
refrigeration?

2) What is the validity, reliability, relevance, and/or experience in
enumerating CD4 on peripheral blood mononuclear cells that have been frozen
and thawed using standard tissue culture techniques, ie, controlled rate
freezing, liquid nitrogen storage, etc.

3) If this has been successfully performed, are literature citations
available?

Apologies if a) this subject is taboo, and/or 2) it has been dealt with
previously in this forum.

Thanks in advance,

Marc Langweiler
Marc.Langweiler@Dartmouth.EDU
Dartmouth-Hitchcock Medical Center
Department of Pathology
Lebanon, New Hampshire 03756
USA
(603) 650-4924 voice
(603) 650-6120 fax

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu