low sort recovery
Alice.L.Givan@Dartmouth.EDU
30 Oct 92 14:42:50 EST
To Lisa Johnson (and other flow networkers),
I think we have got our sorting conditions pretty optimal, although we have
spent a great deal of time trying to convince ourselves of this. But our
recoveries are just about like yours (say 50% of what we think we should
get). The abort frequency that is displayed on the FACStar Plus console is
higher than we think it should be and the number of cells displayed as sorted
on the console is lower than we think it should be -- considering the flow
rate of our cells, the number of drops deflected, the drop drive frequency,
etc.. The best suggestion that we have had so far (from Dave Small at BD) is
that cells may "bunch" together in the nozzle, so that even if the overall
flow rate is low, cells may travel through the nozzle in groups and there
may therefore be more coincidence events than predicted. Perhaps this could
be called the Fifth Avenue Omnibus Effect. We are working on trying to
improve this by diluting cells but pushing them through under greater
pressure. Have you tried this?
Thanks for your help.
Alice Givan
Alice.L.Givan@dartmouth.edu
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