Sort Yields
Alice.L.Givan@Dartmouth.EDU
22 Oct 92 17:13:10 EDT
Here's a problem. We have been sorting and getting very high purity in our
sorted populations. Unfortunately we sometimes get fairly low yields,
relative to the number of cells we start out with. I realize that this is a
familiar problem -- and could involve cells sticking to tubes, cells falling
apart, incorrect counts , etc., etc.. However, when our yields remained low
despite all precautions, we decided to look to see what was remaining in the
cytometer center stream during the sort. What we have found is that very
large numbers of our supposedly sorted cells are not being sorted but go into
the waste. This seems to happen despite very low flow rates and other
conditions that should minimize the aborted sorting decisions. Has anyone
else checked to see how efficient their sorters are at actually removing
selected cells from the original sample? Am I wrong in thinking that, if
the flow rate is slow and the drop delay is correct, the center stream
should contain few, if any, of the cells that have been selected for
sorting?
Any ideas?
Alice Givan
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