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I received from Bob Murphy a PECAN.EXE which signs on as version 1.2.
The file write date is 8/22/88, size 38,594. The source code I
received from Mike Lance is for a version 1.0, 3/15/88, EXE size
38,864. It would be helpful if someone can send me the source code
for the "final" version released by the authors (Morgan P. Conrad and
Ravi Mhatre, who were at B-D in 1988).
I wonder if anyone has already made enhancements, in which case I'd like
to know what has been added and I'd like to be able to start with the
most recent source code available.
Alternatively, if anyone has a "better" or alternative "free" program
for PC analysis of FCS1.0 or FCS2.0 files, I'd like to know more.
*PeCAN (PC ANalysis) is an elegant free program for determination of
percentage positive cells from FCS1.0 files for one, two, or three
color work. It supports polygonal gates, requires EGA, and puts out
summary files in ASCII database format (e.g. suitable for Lotus).
After you have set the gate (typically on FSC vs. SSC or scatter vs.
FL3 = propidium iodide), and set a division line for positive/negative
on each color (it will automatically set the division for 99% negative
on your control cells!), it processes all files in the current
directory without further intervention. However, it displays the dot
plot and histograms for each file for a user-specified number of
seconds during which pressing any key allows modifications.
--
/* - - - - - - - - - - - - - - - - - From - - - - - - - - - - - - - - - - - -
Eric Martz emartz@titan.ucc.umass.edu Professor of Immunology
Voice 413-545-2325, FAX 413-545-1578
Morrill IVN Rm 203, Dept Microbiol, Univ Mass, Amherst MA 01003 USA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - */
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