Re: Wavelength Shift Calibration
Robert F. Murphy (MURPHY@A.CFR.CMU.EDU)
Mon, 13 Jul 1992 15:44:38 EDT
In response to Eric Martz's mail message regarding use of beads
for calibration of ion measurements by flow cytometry, the
first use of that method that I am aware of is described in
the paper "Endosome pH measured in single cells by dual fluorescence
flow cytometry: Rapid acidification of insulin to pH 6" (1984)
J. Cell Biol. 98:1757-1762 (see Figure 1).
For calibration of illuminated volume by a similar method and
a general discussion of discrimination of bound and free ligand
by flow, see Chapter 18 of Flow Cytometry and Sorting, Second Edition,
(Melamed, Lindmo, Mendelsohn, eds.) Wiley-Liss, Inc., 1990, pp. 355-366.
There is also a discussion of the possible effect of AC-coupling
circuits.
Bob Murphy
Carnegie Mellon University
murphy@a.cfr.cmu.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
