Fluorescent tags for free DNA

Robin Barclay (100066.300@CompuServe.COM)
23 Sep 96 04:50:26 EDT

Dear colleagues
We want to tag free DNA to follow its possible delivery into cells (cell lines
or human leucocytes) using flow cytometry (FACSort). We have been tinkering
with sytox green from Molecular Probes, but would welcome any suggestions for
other potential labels. The label should be excitable/readable in the above
system, and stay associated with DNA through a 80C warm-up step.
Robin Barclay
Scottish National Blood Transfusion Service
Edinburgh

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu