Common Mistakes in FACS-based Science & Fantasy
Mario Roederer (Roederer@Darwin.Stanford.EDU)
Wed, 11 Sep 1996 17:14:48 -0800
A student in the lab here decided to do a small presentation to her class on
FACS... basic theories etc.... She asked me for a reference on "common
mistakes" that people make in FACS. I'm not aware of a compendium devoted
solely to such a topic, and thought it might be interesting to put together some
material on this. (Yup, another tutorial Web Page...). If there is Web
material out there, let me know so I can put in links.
Thus, I would like to solicit ideas and examples from the community. There are
the major things, like bad compensation (and what that leads to); more subtle
problems like mistaken inferences from viewing dot plots instead of contour
plots; and related topics like the problems of Cy5 "binding" to monocytes.
I.e., I'm looking for things that a person who is just trying to do a simple
experiment might encounter but wouldn't realize without the years of experience
that an operator might have. General topics might include: sample preparation
(this should only include facets specific to flow cytometry); instrumentation;
data analysis; and data presentation.
Please respond directly to me: Roederer@Darwin.Stanford.Edu. Please send
things you think are obvious (I rarely think of obvious things!), send me your
pet peeves, etc. Unless you specify, anything that results will acknowledge
your contribution.
mr
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
