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PE/Cy5 reagents tend to be brighter, at least in my experience. The big
selling point of PerCP is that it does not require significant FL2-%FL3
compensation. This seems a silly claim to make in that the FL3-%FL2
compensation is always many times greater than the FL2-%FL3, i.e. it
takes a lot more comp to get the PE signal out of my FL3, than to get
the PE/Cy5 out of my FL2 AND this larger compensation is independent of
which FL3 reagent you use. I used a CD4 PE/Cy5 last week which required
a 2% comp.
PerCP has been used on stream in air sorters with generally poor
results, whereas PE/Cy5 works fine.
A final note: Unlike FITC or PE, PE/Cy5 is not purchased from a
manufacturer; each company's chemist is making their own "homemade"
PE/Cy5 conjugate. Thus each will have slightly different compensations
required.
Now when is somebody gonna make me an APC/Cy7 streptavidin conjugate???
Calman Prussin
NIAID/ NIH
>----------
>From: steven micko
>Sent: Wednesday, September 4, 1996 10:02
>To: Cytometry Mailing List
>Subject: CyChrome & PerCP
>
>
>
> Can anyone give me information on the chemical structure (in a general
> way), excitation/emission information, and stability information on the
> two fluorochromes, CyChrome (Pharmingen) and PerCP (B-D)? I'm not
> interested in violating anybody's proprietary information. I just want to
> know what I'm dealing with. So far all I have been able to get is
> marketing stuff (i.e. ours is better than theirs).
>
> Is there anything in the literature?
>
> Thanks in advance.
>
> Steve Micko
> Emory Hospital
> Atlanta
>
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