3-col surface marker/i.c. cytokine staing

Ress, S, Stan, Dr (srress@liver.uct.ac.za)
Sun, 8 Sep 1996 14:26:21 SAST-2

Hi all,

We would like to stain human PBL for simultaneous dual color surface
markers, and IFN gamma intracellular expression, using a coulter
epics profile 2. We would like to quantitate IFN -G production by
memory T cells and CD4/8 subsets in this way. Most of the commercial
mca to surface markers will be FITC/PE combinations, does anyone know
of any available IFN-gamma MCA conjugated to a third fluorochrome
which could be used in these experiments? I understand that for I.C.
work PE should be used, not FITC, but this won't work as PE-conjugated
MCA will be used for surface marker as indicated.
Any advice?

Thanks,

Stan.
Stan Ress
Clinical Immunology laboratory
Dept of Medicine
University of Cape Town
Cape Town, South Africa

e-mail: sress@uctgsh1.uct.ac.za
Phone: Intern + 2721-4066201
FAX : Intern + 2721-4486815

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