protocols for cho cells

Martin, Jill V. (martin.jill@mayo.edu)
Fri, 23 Aug 1996 11:26:33 +0000

We are studying the expression of a protein with several putative membrane
spanning domains using synthetic peptide antibodies. We are interested in
hearing from individuals with experience in FACS analysis of substrate
attached CHO cells, including methods of detachment, suspension,
permeabilization and fixation suitable for FACS analysis.

You may reply to Jill Martin as above, or to me directly:
mcdonald.john@mayo.edu.

Thanks in advance, John McDonald, Mayo Clinic Scottsdale.


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