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Dear Colleagues,
>
>Dean Hewish recommends adding sodium hypochlorite to ethidium bromide
>solutions. However, reference to Sambrook Fritch and Maniatis
>(Molecular Cloning, a lab manual) turned up the following:
>
>"Treatment of dilute solutions of ethidium bromide with hypochlorite
>is not recommended as a method of decontamination. Such treatment
>.... converts the dye into a compound that is mutagenic ....
>Quillardet P and Hofnung M Trends Genetics 4:89 (1988).
The reference I quoted claims that the ethidium is converted into
2-carboxybenxo-phenone and that this is not physiologically active and is
negative in the Ames test. There obviously needs to be some more work done
on this problem.
Dr. Dean R. Hewish,
Cell Biologist & Flow Cytometrist.
CSIRO Biomolecular Engineering,
343 Royal Parade, Parkville, 3052, Victoria
Australia.
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