Re: Platelet activation

Brian Smith (brian.smith@yale.edu)
Mon, 5 Aug 1996 13:39:26 -0400 (EDT)

To: cyto-inbox
In response to your question about measuring in vivo platelet activation, it
can be done. The trick includes (1) careful blood drawing to minimize
artefactual activation, (2) immediate fixation to avoid post-draw
activation. Several markers work, again the important thing is to have a
well-conjugated, "good" antibody (the B-D CD62P is one such antibody). We
have published a number of articles in this area, a few are listed below for
reference. Good Luck!
-Brian
Brian Smith
Yale Medical School
Refs:
Rinder CS, Bonan JL, Rinder HM, Mathew J, Hines R, Smith BR. 1992.
Cardiopulmonary Bypass Induces Leukocyte-Platelet Adhesion. Blood 79:1201-1205.
Rinder CS, Student LA, Bonan JL, Rinder HM, Smith BR. 1993. Aspirin does not
inhibit ADP-induced Platelet -granule Release. Blood 82:505-512.
Rinder CS, Gaal D, Student LA, Smith BR. 1994. Platelet-Leukocyte Activation
and Modulation of Adhesion Receptors in Pediatric Patients with Congenital
Heart Disease undergoing Cardiopulmonary Bypass. J Thorac Cardiovasc Surg
107:280-288.
Rinder HM, Ault KA, Jatlow P, Kosten R, Smith BR. 1994. Platelet
Alpha-granule Release in Cocaine Users. Circulation 90:1162-1167.
Smith BR and Rinder HM. 1996. Interactions of Platelets and Endothelial
Cells with Erythrocytes and Leukocytes in TTP. Sem Hematol (in press).
Rinder CS, Rinder HM, Smith BR, Fitch JCK, Smith MJ, Tracey JB, Matis LA,
Squinto SP, Rollins S. 1995. Blockade of C5a and C5b-9 generation inhibits
leukocyte and platelet activation during extracorporeal circulation. J.
Clin. Invest. 96:1564-1572.
Rinder CS, Mathew J, Rinder H, Bonan J, Ault K, Smith BR. 1991. Modulation
of Platelet Surface Adhesion Receptors During Cardiopulmonary Bypass.
Anesthesiology 75:563-570.
Rinder HM, Bonan J, Rinder CS, Ault KA, Smith BR. 1991. Activated and
Unactivated Platelet Adhesion to Monocytes and Neutrophils. Blood 78: 1760-1769.
Rinder HM, Bonan J, Rinder CS, Ault KA, Smith BR. 1991. Dynamics of
Leukocyte-Platelet Adhesion in Whole Blood. Blood 78:1730-1737.

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